Extended Data Fig. 4: Effects of eliminating permissive CPS from another B. thetaiotaomicron strain. | Nature Microbiology

Extended Data Fig. 4: Effects of eliminating permissive CPS from another B. thetaiotaomicron strain.

From: Phase-variable capsular polysaccharides and lipoproteins modify bacteriophage susceptibility in Bacteroides thetaiotaomicron

Extended Data Fig. 4

Effects of eliminating permissive CPS from another B. thetaiotaomicron strain. a, We identified B. thetaiotaomicron 733056 as the only sequenced and genetically tractable strain that contains VPI-5482-like cps loci (cps2, cps5, and cps6). Gene colors illustrate syntenic genes with >98% amino acid identity but do not indicate function. Please see Extended Data Fig 1 for functional annotation of B. thetaiotaomicron VPI-5482 cps loci. We also observed that the Branch 2 phage SJC01 did not yield productive infection in B. thetaiotaomicron 7330, but could partially clear lawns of B. thetaiotaomicron 7330 at high titers. This ability to clear lawns is a previously described phenomenon known as “lysis from without57”. b, Deletion of permissive capsules (cps2, cps5, and cps6) either alone or in combination affects VPI-5482 infection by SJC01 (n = 7 biological replicates per strain; bars represent the geometric mean). c, While SJC01 plaques on WT B. thetaiotaomicron VPI-5482, it does not form plaques on wild-type B. thetaiotaomicron 7330. However, SJC01 does exhibit a “lysis from without” clearing phenotype at high densities of phage (top two spots, made with 1 microliter of 1e8 and 1e7 PFU per mL, according to titers observed on wild-type VPI-5482). Experiment was independently performed 7 times with VPI-5482 and 8 times with 7330 with similar results. d, B. thetaiotaomicron 7330 strains lacking cps5 (with the exception of 7330 ∆cps5 ∆cps6) show the lysis from without phenomenon less frequently than strains that have intact cps5. Experiments were performed with the following number of replicates per strain with similar results: wild-type 7330 (n = 8), Δ2 (n = 8), Δ5 (n = 8), Δ6 (n = 8), Δ2,5 (n = 17), Δ2,6 (n = 5), Δ5,6 (n = 3), Δ2,5,6 (n = 19). For panel b, significant differences in phage titers on each mutant strain were compared to wild type via two-tailed Mann-Whitney test with actual P values shown. For panel c, scale bars = 0.5 cm.

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