Extended Data Fig. 6: Infection of wild-type B. thetaiotaomicron at a low multiplicity of infection and subsequent effects on cps gene expression.
Infection of wild-type B. thetaiotaomicron at a low multiplicity of infection and subsequent effects on cps gene expression. a, The wild-type (WT) strain was infected at a low multiplicity of infection (MOI = 1 ×10-4) of live or heat-killed ARB25, and bacterial growth was monitored via OD600 (n = 3 biological replicates and separate curves are shown). b, RNA was harvested from cultures after reaching an OD600 of 0.6-0.7, cDNA was generated, and relative expression of the 8 cps loci was determined by qPCR (histogram bars are mean ± SEM of 3 biological replicates). Individual replicates of high MOI (c) and low MOI (d). In the high MOI experiment, replicate 2 showed higher starting expression of the non-permissive CPS3 compared to others. In the low MOI experiment, replicate 3 showed higher starting expression of the non-permissive CPS3. In both experiments, post phage-exposed replicates displayed nearly identical CPS expression profiles characterized by high expression of CPS3. The experiments in a-c were repeated one time with three parallel biological replicates started from single B. thetaiotaomicron wild-type colonies picked from the same plate.
