Extended Data Fig. 10: ARB25 (a) or SJC01(b) infection of the acapsular BT1927 locked on and off strains after 1, 2 or 3 days of growth on BPRM.
ARB25 (a) or SJC01(b) infection of the acapsular BT1927 locked on and off strains after 1, 2 or 3 days of growth on BPRM (n = 6 biological replicates per treatment, performed with similar results). Three separate colonies were picked each day as a biological replicate, grown overnight and used to setup infection cultures that were monitored for 24 h in an automated plate reader. Colonies picked after only 1 day show the least resistance to either phage when BT1927 is locked on. After 2 days, resistance is increased and this continues to increase after 3 days, becoming almost complete (compared to HK controls for ARB25). Growth curves represent the mean of 6 biological replicates ± standard error.
