Extended Data Fig. 3: Replication of a subset of host range assays of B. thetaiotaomicron-targeting phages on strains expressing different CPS types.
Replication of a subset of host range assays of B. thetaiotaomicron-targeting phages on strains expressing different CPS types. Ten bacteriophages isolated and purified on the wild-type, acapsular, or the 8 single CPS-expressing strains were re-tested in a spot titer assay to determine phage host range. 10-fold serial dilutions of each phage ranging from approximately 106 to 103 plaque-forming units (PFU) / ml were spotted onto top agar plates containing the 10 bacterial strains. Plates were then grown overnight, and phage titers were calculated. Titers are normalized to the titer on the preferred host strain for each replicate. Each row in the heatmap corresponds to a replicate for an individual phage, whereas each column corresponds to one of the 10 host strains. One to three replicates of the assay were conducted for each phage by the two lead authors (AJH and NTP). Assays were carried out at the same time, and each author used the same set of cultures and phage stocks. For comparison, individual replicates from Fig. 1 are included (marked with *). Experiment was conducted once during a visit of AJH to the University of Michigan research site to compare reproducibility between experimenters with the replication described above.
