Extended Data Fig. 8: Detailed characterization of the NOT gates.

These data correspond to Fig. 5b and Supplementary Table 2. The detailed schematic for the gate is shown to the left and the corresponding strains are provided in Supplementary Table 4. The sequences for the genetic parts are provided in Supplementary Tables 4 and 8. The data used to fit the response functions (Equation 2) were calculated as described in Extended Data Figure 6 and the resulting parameters are provided in Supplementary Table 2. The data represent three experiments performed on different days. The experimental conditions are as described in the Methods. Two representative cytometry distributions are shown when cells containing the gates are grown in 0 or 20 mM IPTG. The dynamic data was measured as described in the Methods and fit to Equations 6, 7 and 8. The data represent two experiments performed on different days.