Extended Data Fig. 10: The impact on growth from carrying the circuits in different states.

Each point represents a different combination of inducers for a given circuit. The prediction is made using Cello and is calculated by multiplying the empirically-measured growth impact of all the gates with input promoter activities corresponding to that state [7]. Details regarding the growth assay are presented in the Methods and normalized to S. cerevisiae BY4741 in the same growth conditions. The data represent three experiments performed on different days.