Extended Data Fig. 1: Optimization steps for building the TetR-responsive minimal promoter.
The annotated promoter sequences are provided in Supplementary Table 5, including intermediates, and all part sequences are provided in Supplementary Table 10. The promoters are evaluated through the transcriptional fusion with yfp and this cassette is carried on a plasmid (Supplementary Figure 15). The data are measured under identical experimental conditions (Methods). In the top graph, the carbon source is either 2% glucose or 2% galactose. The star indicated that there is no fluorescence detected over background. When TetRNLS is expressed from a second plasmid (Supplementary Figure 15), this is indicated by “+tetRNLS cassette”). The red arrows show the data corresponding to the promoter selected for the next round of optimization. The horizontal orange lines mark where mutations are made in order to diversify the part sequence to avoid homologous recombination in the context of a circuit. The data represent the average of three experiments performed on different days. The optimizations steps for other promoters are shown in Supplementary Figs. 2–12.
