Extended Data Fig. 2: Promoter designs with varying lacO_s operator spacing.

The sequences of genetic parts are provided in Supplementary Table 5. The UAS binds to Gal4 and the TSS is a 20bp sequence from ADH2 (Extended Data Figure 1). The promoters are transcriptionally fused to yfp and cloned into a plasmid backbone (Supplementary Figure 15). A second plasmid is constructed where LacI is expressed from a constitutive promoter (Supplementary Figure 15). The cytometry distributions show the fluorescence from the reporter plasmid in the absence (black) and presence (grey) of the LacI plasmid and the reported “Repression” is the ratio of the medians of these distributions. The data represent the average of three experiments performed on different days.