Extended Data Fig. 6: Ly6eΔHSC mice are susceptible to mouse hepatitis virus.
From: LY6E impairs coronavirus fusion and confers immune control of viral disease
a, b, Male Ly6efl/fl and Ly6eΔHSC mice injected with PBS or MHV and monitored for survival (a) and weight loss (b). c–n, Mice were euthanized at 3- (c–h) or 5- (i–n) days post-infection for determination of serum ALT (c,i), viral titers in liver (d,j) and spleen (e,k), and liver necrosis and inflammation (f–h, l–n). For a, b, n=15 for days 0-6, n=14 for days 6-14, (Ly6efl/fl), n=10 for days 0-5, n=4 for days 5-6, n=0 for days 6-14 (Ly6eΔHSC) from three pooled experiments. For c–g, n=8 (Ly6efl/fl,), n=6 (Ly6eΔHSC), or n=6 (PBS) from two pooled experiments; images shown in h are representative of the same cohorts. For i–m, n=8 (Ly6efl/fl,), n=6 (Ly6eΔHSC), or n=3 (PBS) from two pooled experiments; images shown in n are representative of the same cohorts. In h, n, scale bars are 500 µM (4x) and 100 µM (20x). Significance for was tested by two-sided Mantel-Cox test (a), two-tailed unpaired student’s t-test with Welch’s correction (c–e, i–k), two-tailed Mann-Whitney U test (f, g, l, m). Data are presented as mean values ± SEM (b) or SD (c–g, i–m). P value: a, **** p=3.946 x 10-6; e, ** p=0.0026; k, * p=0.0119.
