Extended Data Fig. 7: Ly6eΔHSC mice have enhanced sensitivity to mouse hepatitis virus and a differential gene expression profile.
From: LY6E impairs coronavirus fusion and confers immune control of viral disease
a–j, Female (a–e) and male (f, g) Ly6efl/fl and Ly6eΔHSC mice injected with PBS or MHV and assessed for serum ALT (a, f), viral titers in liver (b, g) and spleen (c, h), and liver necrosis and inflammation (d, e, i, j). k–n, Female Ly6efl/fl and Ly6eΔHSC mice injected with PBS or MHV and whole livers were imaged. Mock-infected (PBS) Ly6eΔHSC mice (k), mock-infected Ly6efl/fl mice (l), MHV-infected Ly6eΔHSC mice (m), MHV-infected Ly6efl/fl mice (n). o, p, Representative genes from transcriptomic analysis of liver (o) and spleen (p) from MHV-infected and PBS-injected female Ly6efl/fl and Ly6eΔHSC mice. For a–e, n=8 (Ly6efl/fl,), n=8 (Ly6eΔHSC), or n=3 (PBS) from two pooled experiments per sex. For f–j, n=9 (Ly6efl/fl,), n=7 (Ly6eΔHSC), or n=3 (PBS) two pooled experiments. For k–p, n=3. Statistical significance was determined by two-tailed unpaired student’s t-test with Welch’s correction (a–c, f–h), two-tailed Mann-Whitney U test (d, e, i, j). Data are presented as mean values ± SD (a–j; o, p). P value: a, * p=0.0234; b, * p=0.0428; c, *** p=0.0004; d, * p=0.0210; e, ** p=0.0054.
