Fig. 3: Ly6e^ΔHSC mice are more susceptible to MHV.

a,b, Female Ly6e^fl/fl and Ly6e^ΔHSC mice were injected with PBS or 50,000 p.f.u. MHV-A59 and monitored for survival (a) and weight loss (b). c–n, Mice were assessed at 3 (c–h) or 5 d post-infection with 50,000 p.f.u. MHV-A59 (i–n) for serum ALT (c and i), viral titres in the liver (d and j) and spleen (e and k), liver necrosis (f and l) and inflammation (g and m). Images of liver sections demonstrating necrosis inflammation are shown in h and n. In a and b, n = 13 for days 0–14 (for Ly6e^fl/fl), and n = 11 for days 0–3, n = 10 for days 4–5, n = 5 for day 6 and n = 3 for days 7–14 (for Ly6e^ΔHSC), from three pooled experiments. In c–g, data represent the means of n = 9 (Ly6e^fl/fl), n = 10 (Ly6e^ΔHSC) and n = 6 mice (PBS), from two pooled experiments. The images in h are representative of the same cohorts. In i–m, data represent the means of n = 9 (Ly6e^fl/fl), n = 7 (Ly6e^ΔHSC) and n = 6 mice (PBS), from two pooled experiments. Images in n are representative of the same cohorts. In h and n, scale bars are 500 µM (4×) and 100 µM (20×). Statistical significance was determined by two-sided Mantel–Cox test (a), two-tailed unpaired Student’s t-test with Welch’s correction (c–e and i–k) and two-tailed Mann–Whitney U-test (f, g, l and m). Data are presented as means ± s.e.m. (b) or s.d. (c–g and i–m). P values: ***P = 0.0002 (a); **P = 0.0031 (c); ****P = 5.759 × 10⁻⁷ (e); **P = 0.0054 (g); *P = 0.0430 (i); **P = 0.0094 (k); **P = 0.0072 (l); and ****P = 8.741 × 10⁻⁵ (m).