Extended Data Fig. 5: Generation of hematopoietic Ly6e conditional knockout mouse.
From: LY6E impairs coronavirus fusion and confers immune control of viral disease
a, Strategy used to generate Ly6eΔHSC mice. Hematopoietic-tissue specific ablation of Ly6e was achieved by crossing Ly6efl/fl mice with transgenic Vav1-iCre mice to remove the LoxP-flanked exon III and IV, resulting in a 17 amino acid truncated variant of the 130 amino acid full-length protein. Primers used for tissue genotyping and gene expression are also depicted as arrows. b, Gel electrophoresis of tissue genotyping PCR, representing Ly6efl/fl, Ly6efl/+, and Ly6e+/+ mice. c, Ly6e gene expression in bone marrow-derived macrophages (BMDM). d, Infection of BMDM. For c-d, n=9 (Ly6efl/fl) or n=8 (Ly6eΔHSC) mice from three pooled experiments. The gel shown in b is representative of all genotyping performed to identify Ly6efl/fl mice. Data for c, d is shown normalized to Ly6efl/fl values. Statistical significance was determined by using two-tailed unpaired student’s t-test with Welch’s correction (c) or two-tailed ratio-paired t-test (d). Data are presented as mean values ± SD (c-d). P values: c, *** p=0.0010. d, ** p=0.0021.
