Extended Data Fig. 7: Analyses of AcaB oligomerisation in solution. | Nature Microbiology

Extended Data Fig. 7: Analyses of AcaB oligomerisation in solution.

From: Comprehensive analysis of IncC plasmid conjugation identifies a crucial role for the transcriptional regulator AcaB

Extended Data Fig. 7

a, SEC-MALS. Red and blue curves indicate the normalised UV traces of the WT protein and the E69A mutant, respectively. The dotted lines under the peaks are calculated average molecular mass using the same colour scheme. b, Glutaraldehyde and SDS-PAGE. Lane 1: AcaB with 0 % (v/v) glutaraldehyde; Lane 2: AcaB with 0.005 % (v/v) glutaraldehyde; Lane 3: AcaBE69A with 0 % (v/v) glutaraldehyde; Lane 4: AcaBE69A with 0.005 % (v/v) glutaraldehyde. Each experiment was repeated three times with similar results.

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