Table 1 General features of the manually curated phage genomes

From: Large freshwater phages with the potential to augment aerobic methane oxidation

Sampling site

Sampling year

Genome name (short name)

Length (bp)

GC content (%)

No. of ORFs

No. of tRNAs

Complete or partial

pmoC taxonomy

BML (Canada)

2015–2017

BML_pmoC-phage_2 (BML_2)

218,687

33

342

15

Partial

Gamma-

BML_pmoC-phage_3 (BML_3)b

190,971

34

272

20

Partial

Gamma- c,d

BML_pmoC-phage_4 (BML_4)

243,619

34

342

18

Complete

Gamma-c

BML_S (Canada)

2017

BML_S_phage_1 (BML_S_1)

167,437

40

212

24

Complete

TP6 (Canada)

2012

TP6_pmoC-phage_1 (TP6_1)

308,538

37

406

29

Partial

Gamma-c

PDSYNTPWS (Canada)

2012

PDSYNTPWS_phage_1 (PDSYNTPWS_1)b

222,435

34

358

20

Partial

Lake Mendota (Madison, WI, United States)

2008–2012

Lake_Mendota_pmoC-phage_1 (LM_1)

174,291

41

249

21

Complete

Alpha-

Lake_Mendota_pmoC-phage_2 (LM_2)

174,276

39

263

24

Complete

Alpha-

Lake_Mendota_pmoC-phage_3 (LM_3)

172,382

41

249

21

Complete

Alpha-

Lake_Mendota_pmoC-phage_4 (LM_4)

353,177

32

465

14

Complete

Gamma-

Lake_Mendota_pmoC-phage_5 (LM_5)

166,198

38

245

19

Complete

Alpha-

Lake_Mendota_pmoC-phage_6 (LM_6)b

198,907

34

313

20

Partial

Gamma-

Lake_Mendota_phage_7 (LM_7)

166,826

39

238

25

Complete

Lake_Mendota_pmoC-phage_8 (LM_8)

167,952

39

252

25

Complete

Alpha-e

Lake_Mendota_phage_9 (LM_9)

172,107

40

240

22

Complete

Crystal Bog (Madison, WI, United States)

2007–2009

Crystal_Bog_pmoC-phage_1 (CB_1)

352,383

35

445

23

Complete

Alpha-

Crystal_Bog_pmoC-phage_2 (CB_2)

527,138

38

594

13

Complete

Alpha-

Crystal_Bog_pmoC-phage_3 (CB_3)

166,456

35

247

18

Complete

Gamma-

Crystal_Bog_pmoC-phage_4 (CB_4)

165,508

38

264

4

Complete

Alpha-

Crystal_Bog_pmoC-phage_5 (CB_5)

166,149

44

248

4

Complete

Alpha-d

Crystal_Bog_phage_6 (CB_6)

174,375

38

233

0

Complete

Lake Rotsee (Switzerland)

2017–2018

Lake_Rotsee_pmoC-phage_1 (LR_1)

168,581

40

224

4

Complete

Alpha-

Lake_Rotsee_pmoC-phage_1 (LR_2)

160,734

37

241

6

Complete

Alpha-

Lake_Rotsee_pmoC-phage_1 (LR_3)

159,173

35

248

10

Complete

Gamma-

Lake_Rotsee_pmoC-phage_1 (LR_4)

365,676

36

467

4

Complete

Gamma-

Lake_Rotsee_pmoC-phage_1 (LR_5)

341,475

36

463

15

Complete

Gamma-

Lake_Rotsee_pmoC-phage_1 (LR_6)

314,403

38

442

0

Complete

Gamma-c

  1. aThe taxonomy is determined based on pmoC phylogeny including both phage and bacterial pmoC genes. bHighly similar phage genomes, with identical large terminase and DNA polymerase sharing >99.5% amino-acid similarity. cFragmented pmoC. dSome cells within the population only have partial pmoC. ePartial pmoC.