Extended Data Fig. 4: Transcriptional changes induced by purified cell wall components and their respective exposure on C. albicans and C. auris surface.
a, Heatmap displaying the Log2Fold change (color scale) of the top 50 DEG of C. albicans live, for both Candida species and their cell wall components, β-glucan and mannan, at 4 h (left panel) and 24 h (right panel). b, Flow cytometry plot based on forward scatter component (FSC) and side scatter component (SSC), demonstrating C. auris strains are slightly smaller and of higher complexity than C. albicans. c, Flow cytometry-based comparison of cell wall components of C. albicans and C. auris strains. Mean fluorescent intensity (MFI) of thimerosal-fixed Candida cells stained for Fc-Dectin-1, a marker for β-glucan (left), and ConA, a marker for mannans (right). Graphs represent mean ± SEM of the 3 means, each performed with three replicates in three independent measurements, * p < 0.05, Kruskall Wallis test with two-sided Dunn’s multiple comparison test was performed comparing the respective C. auris strains with the two C. albicans reference strains. Data used to make this figure can be found in Source Data Extended Data Fig. 4.
