Extended Data Fig. 1: Transcriptomic profiling PBMCs stimulated with live C. albicans or C. auris and respective cell wall components β-glucans and mannans for 4 and 24 hours.
a, Principal component analysis (PCA) performed on normalized count data (normTransform, DESeq2) demonstrates the main component introducing variance in the dataset is time (40%), as indicated by a clear split between the early 4-hour host induced response (left, triangle) and the late 24-hour response (right, circle). To a lower extent (15%), the second component introducing variance appears to be inherent to the stimulus (color). b, At 4 h, PCA reveals a clear donor clustering (shape) irrespective of stimulus (color), indicating the main variance in the early host response reflects inter-individual differences (left). PCA on the late response, 24 h, is predominantly influenced by the respective stimulus (38%, color), and to a lower extent by the donors (19%, shape), indicated by the scattering of stimuli together with a rough clustering amongst donors. c, Pathway enrichment plot displaying the top 20 enriched pathways for both C. albicans live and C. auris live (color) at 24 h. Enrichment determined using Consensus PathDB, including pathways as defined by KEGG and Reactome (shape), considering a p-adjusted value < 0.01 (indicated as ‘q-value’) significant. Size of the geometric points indicates the amount of DEG in relation to the pathways’ size. The exact q values and the data used to make this figure can be found in Source Data Extended Data Fig. 1.
