Extended Data Fig. 1: Synthesis, efficacy, and photolysis of the fluoxazolevir-diazirine-biotin probe.
a, The general synthetic scheme of the fluoxazolevir-diazirine-biotin (fluoxazolevir-DB) probe is shown. Each intermediate was confirmed with 1H NMR and LCMS. See supplemental document for more information on each synthetic step. b, Fluoxazolevir-DB probe retains anti-HCV activity in vitro and shows inhibition against HCV infection in a dose-dependent manner. Data are presented as mean values ± SEM of 6 biologically independent replicates. c, The degradation of fluoxazolevir-DB via UV irradiation is shown. d, The fluoxazolevir-DB was exposed to UV irradiation with a 100 W mercury lamp with a 365 nm bypass filter. Disappearance of fluoxazolevir-DB was measured over time via LCMS and underwent a complete conversion to the carbene insertion product within 10 min. All results are representative of three independent experiments.
