Extended Data Fig. 2: Dose-response curves of fluoxazolevir against amplified HCV from the in vitro drug resistance selection assay.
Among the 8 serial passages with potential RAS-containing HCV generated from the drug resistance selection assay (Fig. 2a), the viruses in the following wells (and their identified mutations) showed moderate resistance with EC50 values increasing by at least two-fold comparing to the wild-type control: A1 (F291L, V414A), B1 (I374T), C1 (D382E, T395A, M405V, P616A), E1 (F291V), G1 (A274S) and H1 (M267V, V284A). The same viruses were tested against sofosbuvir as a control and were equally sensitive to sofosbuvir as the wild type virus. Data are presented as mean values ± SEM of 3 biologically independent replicates. All results are representative of three independent experiments.
