Extended Data Fig. 3: Viral fitness of the generated RAS-containing HCV. | Nature Microbiology

Extended Data Fig. 3: Viral fitness of the generated RAS-containing HCV.

From: Fluoxazolevir inhibits hepatitis C virus infection in humanized chimeric mice by blocking viral membrane fusion

Extended Data Fig. 3

a, The viral fitness assay scheme is shown here. Huh7.5.1 cells were electroporated with the RNA of each HCV RAS-RLuc construct. b, The first part of the assay assesses the replication capacity for each RAS-containing HCV. Luminescence was measured 4 h and 3 days after electroporation and the readings obtained 4 h after electroporation was used as background. c, The second part of the assay assesses infectivity of each RAS. Viral medium harvested 3 days after electroporation from part b was used to reinfect 104 naïve Huh7.5.1 cells in a 96-well plate. Luminescence was measured 48 h after reinfection and all measurements were normalized to HCV-WT. Data are presented as mean values ± SEM of 3 biologically independent replicates. All results are representative of three independent experiments.

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