Extended Data Fig. 4: Dose-response curves of fluoxazolevir against HCV mutants with putative RASs in core, E1 and E2 regions. | Nature Microbiology

Extended Data Fig. 4: Dose-response curves of fluoxazolevir against HCV mutants with putative RASs in core, E1 and E2 regions.

From: Fluoxazolevir inhibits hepatitis C virus infection in humanized chimeric mice by blocking viral membrane fusion

Extended Data Fig. 4

Huh7.5.1 cells in 96-well plates were infected with wild-type HCV-RLuc (GT 2a) and HCV-RLuc mutants with various putative RASs (R9G, V140L, M267V, A274S, V284A, F291L, F291V, I374T, D382E, T395A, M405V, V414A and P616A) in the presence of various fluoxazolevir concentrations as indicated. Cells were harvested 48 h after infection and luminescence assessed via the luciferase assay. The EC50 values for wild-type HCV-RLuc (black circles) and the HCV mutants (red squares) were calculated with Prism 7. Data are presented as mean values ± SEM of 8 biologically independent replicates. All results are representative of three independent experiments.

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