Extended Data Fig. 8: Applied gating strategies across flow cytometry experiments for epithelial cells. | Nature Microbiology

Extended Data Fig. 8: Applied gating strategies across flow cytometry experiments for epithelial cells.

From: Candida pathogens induce protective mitochondria-associated type I interferon signalling and a damage-driven response in vaginal epithelial cells

Extended Data Fig. 8

a, A431 cells (linked to Fig. 4e) and (b) primary vaginal cells (linked to Extended Data Fig. 6c). First, 104 events were analyzed based on their side scatter area (SSC-A) vs. forward scatter area (FSC-A). For further analysis, single cells were selected based on forward scatter height (FSC-H) vs. forward scatter area (FSC-A). MitoTracker® Deep Red FM signal was measured using detection channel Alexa 647-A. The unstained population was taken as a reference to determine the median fluoresce intensity of all samples (depicted as histogram Alexa 647-A- and Alexa 647-A+). The ratio from the median intensity of the stained/uninfected cells and unstained/uninfected cells was used as a reference to obtain the results of the infected samples shown in the manuscript figures.

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