Extended Data Fig. 9: Pbp2B localization and FDAA incorporation in ∆ZBPs cells.
a Z rings (left) and Pbp2B localization (right) (epifluorescence images of cells expressing both Pbp2B-mNeonGreen and FtsZ-HaloTag induced with 20 µM IPTG for 2 hours and labelled with 5 nM JF549-HTL) in control and ∆ZBPs cells. White arrows indicate the Z ring positions in each image. Representative images from at least two replicates of each condition. Scale bars: 2 µm. b Z rings and fluorescent D-amino acid (FDAA) incorporation (epifluorescence images of cells labelled with 1 mM fluorescent D-lysine (FDL) for 30 seconds, right) in control and ∆ZBPs cells. White arrows indicate the Z ring positions in each image. Representative images from at least 4 replicates of each condition. Scale bars: 2 µm. c Z ring width versus Pbp2B recruitment. Pbp2B intensity at midcell is higher when Z rings are more condensed; this is expected given that Pbp2B recruitment and Z ring condensation both increase over time. N = 2761 Z rings. d Pbp2B directional motion is seen at Z rings of all widths. Z ring width distributions of all Z rings (solid lines) and the Z rings at which Pbp2B moves directionally (dashed lines) for either control cells (left) or ∆ZBPs cells (right) are similar. This confirms that the Pbp2B motion seen in ∆ZBPs is present at decondensed rings. N>100 Z rings in each distribution. Shaded area: SEM.
