Extended Data Fig. 10: FtsA modulates FtsZ dynamics and Z ring formation.
a Z ring morphology (epifluorescence images of cells expressing FtsZ-mNeonGreen induced with 30 mM xylose for 2 hours) in control cells and ∆ftsA cells. ∆ftsA cells have highly altered Z rings. In ∆ftsA cells, FtsZ is expressed with 10 µM IPTG from the pHyperSpank promoter; higher or lower expression levels do not allow for cell survival. Representative images from three replicates of each condition. Scale bars: 2 µm. b Distributions of α values for FtsZ motion in control and ∆ftsA cells, obtained by tracking FtsZ filament motion and fitting each track to MSD(∆t) = D*∆tα. α > 1 indicates directional motion, so FtsZ filaments in ∆ftsA cells exhibit less directional treadmilling compared to control cells. N>6000 tracks for each condition. c Tracks of FtsZ filament motion in control and ∆ftsA cells. Tracks with α > 1 are cyan, tracks with α ≤ 1 are magenta. In control cells, FtsZ filaments often treadmill directionally along the short axis of the cell. In ∆ftsA cells, directional motion occurs less frequently and follows the short axis of the cell less consistently. Segmented cells are shown in black on a grey background. Scale bars: 2 µm.
