Extended Data Fig. 2: The relative abundance of A. muciniphila correlates with iBAT temperature and GLP-1 secretion and A. muciniphila administration to HF-diet-fed mice increases the M2 macrophage count in adipose tissue.
a, Differences in the relative abundance of bacterial species between the HF and HF + Akk groups are represented by ALDEx2. The differences in abundance between and within each group for individual species were analyzed. Organisms (at the OTU and nearest neighbor species levels) with significant p values are shown as pink circles (Welch’s t-statistic, corrected using the Benjamini-Hochberg method). b, Data showing the 16S rRNA gene count in the HF and HF + Akk groups. c–e, Scatter plots illustrating the statistical relationship (Spearman’s correlation) between the relative abundance of A. muciniphila and metabolic phenotypes: OGTT AUC (oral glucose tolerance test area under the curve), iBAT temp (interscapular brown adipose tissue temperature), and GLP-1 (plasma glucagon-like peptide-1 concentration). f–h, mRNA expression of M1 and M2 macrophage markers in iBAT, igWAT, and epiWAT. i, Gating strategy for detection of M2-like macrophages (CD11b+ CD206+) in iBAT of HF-fed mice. Data are presented as the means ± SEMs. Number of mice per group for a–h: HF: 10, HF + Akk: 10. Data in f–h were analyzed using the two-tailed unpaired t-test.
