Extended Data Fig. 5: Intraperitoneal administration of P9 improves glucose homeostasis and body mass in normal chow (NC) diet-fed mice.
a, Weight gain was measured in NC-fed mice administered candidate proteins intraperitoneally (i.p.) (P1, P5, P9, or Amuc_1100) at 100 μg per mouse for 2 weeks. b, Oral glucose tolerance testing (OGTT) was performed and the AUCs were calculated. The data in c represent the effects of P9 derived from A. muciniphila and EcPrc (S41 family derived from E. coli) on GLP-1 secretion by NCI-H716 cells. d, Plasma GLP-1 levels were measured after the single i.p. injection (300 μg per mouse) of each respected group. Data are presented as the means ± SEMs. Number of mice per group for a and b: NC: 8, P1: 8, P5: 8, P9: 8, Amuc_1100: 8 and for d, NC: 8, ErPrc: 8, Acetate: 8, Propionate: 8. Data were analyzed using one-way ANOVA, followed by Tukey’s post-hoc test for a, d. For b, data were analyzed using two-way ANOVA, followed by Tukey’s post-hoc test. The data in c are the results of duplicate experiments and were analyzed using the Kruskal-Wallis test, followed by Dunn’s post-hoc test. * or φ indicate significant differences, P < 0.05.
