Extended Data Fig. 7: The plant-fungus interaction zone visualized by EM.
From: A cell surface-exposed protein complex with an essential virulence function in Ustilago maydis
a, Silver-enhanced immunogold labeling of Stp1-HA and Stp3-HA in biotrophic hyphae. Immuno-labeled hyphal tips inside epidermal plant cells are viewed with transmission electron microscopy. Images confirm specificity of the antibody (no signal in SG200 hyphae) and presence of signals in SG200Δstp1 Stp1-HA and SG200Δstp3 Stp3-HA. Particle size varies with silver enhancement time. b, Cross sections of active fungal hyphae in plant cells visualized by EM. Strains are indicated on the left. Filled arrows indicate electron-dense structures. Open arrows indicate empty vesicular structures. Note that the interaction zone in wild-type infections includes electron-dense structures that are approximately 50 nm. Scale bars, 200 nm.
