Extended Data Fig. 8: Biotrophic growth of SG200Δcomplex cannot be rescued in co-infections with haploid strains.
From: A cell surface-exposed protein complex with an essential virulence function in Ustilago maydis
a, SG200Δcomplex-Cmu1-mCherry (red) was infected alone or co-infected with either FB1 or FB2 and analysed by confocal microscopy as indicated. Growth of SG200Δcomplex-Cmu1mCherry could not be rescued by co-infections with FB1 or FB2. This illustrates that these haploid cells are unable to fuse with SG200Δcomplex-Cmu1-mCherry. Shown are maximum projections of confocal z-stacks. Scale bars, 50 µm. b, Maize seedlings were infected with the indicated strains. 12 d.p.i. disease symptoms were scored using the colour code shown on the right. Data represent mean of n = 3 biologically independent experiments. Total numbers of infected plants are indicated above the respective columns.
