Fig. 2: The Stp complex localizes to speckles on the surface of fungal hyphae.
From: A cell surface-exposed protein complex with an essential virulence function in Ustilago maydis
a, SG200 derived strains expressing the indicated fusion proteins growing in the epidermal layer at 2 d.p.i. mCherry signal, red. Images represent maximum projections of confocal z-stacks. Scale bars, 5 μm. Insets show cross-sections of fungal hyphae. b, Hyphal tip of SG200 derived strain expressing the indicated fusion proteins growing in the epidermal layer at 2 d.p.i. Yellow fluorescent protein (YFP) signal, yellow; mCherry signal, blue. Signal overlay (white) is shown on the right. Images represent maximum projections of confocal z-stacks. Scale bars, 5 µm. c, Left panel shows hyphae of AB33complexcon and AB33 immunostained with anti-HA primary antibodies and AF488 conjugated secondary antibody (green) without prior permeabilization. Right panel shows an overlay with differential interference contrast (DIC). Scale bars, 5 µm. d, Silver-enhanced immunogold labelling of SG200Δstp1-Stp1-HA, SG200Δstp3-Stp3-HA and SG200 in biotrophic hyphae. Transmission electron microscopy images of immunolabelled hyphal tips inside epidermal plant cells. Images confirm specificity of the antibody (no signal in SG200 hypha), and the label is in clusters about 50 nm across. Arrows indicate labelled particles. Particle size varies with silver enhancement time. Scale bars, 400 nm.
