Extended Data Fig. 1: Impact of assembly methods on viral recovery from gut metagenomes.
From: Metagenomic compendium of 189,680 DNA viruses from the human gut microbiome
The MGV catalogue was formed using metagenomic viral contigs identified from three studies that performed large-scale assembly of human stool metagenomes. The CIBIO and MGnify studies used MetaSPAdes for metagenomic assembly while the JGI study used MEGAHIT. To explore the effect of assembler on virus identification, we compared viral contigs identified from a common set of 752 stool samples which were assembled by all three studies and were each represented by a single SRA run accession. a, The number of vOTUs represented by viral contigs (>50% completeness) from each of the three studies. A similar number of vOTUs were identified from metagenomic contigs assembled by each study. b, The number of viral contigs at different quality levels identified from each of the three studies. A greater number of complete and high-quality viral genomes are recovered from the MEGAHIT assemblies.
