Extended Data Fig. 6: Tse8 is not active on a substrate of the amidase Pam.
MS analysis of Tse8 (a) or Pam (b) enzymatic assay using epinicedin-1 as substrate. The antimicrobial peptide epinecidin-1, as well as sermorelin, have amidated C termini. The latter has previously been used to measure the amidase activity of Pam from S. maltophilia55. In both (a) and (b): Sequence covered by the fragments obtained after fragmentation of epinecidin-1 in the MS is indicated above the fragmentation spectra for epinecidin-1. Signals corresponding to the amidated (a) or deamidated (b) form of epinecidin-1 are shown in the spectral plot with red ions belonging to the b series of fragments, blue ions to the y series, and green ions to parental forms of the peptide. See Extended Data Fig. 7 for correspondence between the observed fragments and their theoretical masses.
