Extended Data Fig. 3: Prey killing is mediated by Tse8 and effects can be complemented by expressing Tse8 or Tsei8 in trans.
a, In the absence of Tse8 (PAKΔretSΔtse8) or the H1-T6SS (PAKΔretSΔH1) there is no reduction in recovered recipient (PAKΔretSΔtsei8) as occurs when the donor has a fully active T6SS (PAKΔretS). b-c, The PAKΔretSΔtsei8 (b) or PAKΔretSΔtse8 (c) mutation can be complemented in trans. Competition assays were performed with donors PAKΔretS or PAKΔretSΔH1 and recipient PAKΔretSΔtsei8 with either empty pBBR1MCS5 or the complementation vector pBBR1:tsei8 (b) or recipient PAKΔretSΔtsei8 with either empty pBBR1MCS4 or the complementation vector pBBR1:tse8 (c). Statistical analyses: a, Mean CFUs/mL ± SEM of recipient cells in competition/alone are represented from represented from three independent replicates performed in triplicate (n=3). Two-tailed student’s t-test, * p<0.05 for PAKΔretS [pBBR1-MCS4] vs recipient compared to PAKΔretSΔtse8 [pBBR1-MCS4] vs recipient, PAKΔretSΔtse8 [pBBR1-MCS4] vs recipient compared to PAKΔretS [pBBR1:tse8] or PAKΔretSΔtse8 [pBBR1:tse8] vs recipient. b, Mean CFUs/mL ± SEM of recipient cells in competition/alone are represented from represented from five independent replicates performed in triplicate (n=5). Two-tailed student’s t-test, ** p<0.005 compared to PAKΔretS donor vs recipient PAKΔretSΔtsei8 [pBBR1-MCS5] compared separately to the other datasets; ns between recovered CFUs/mL for recipient PAKΔretSΔtsei8 [pMMB-MCS5] vs PAKΔretSΔH1 (p=0.51) and recipient PAKΔretSΔtsei8 [pMMB:tsei8] vs PAKΔretS (p=0.61). c, Mean CFUs/mL ± SEM of recipient cells in competition/alone are represented from represented from three independent replicates performed in triplicate (n=3). Two-tailed student’s t-test, ** p<0.005 for PAKΔretS donor vs recipient compared separately to the other datasets.
