Fig. 3: Characterization of important sequence features of the AZD8895 public clonotype.
From: Genetic and structural basis for SARS-CoV-2 variant neutralization by a two-antibody cocktail
a, IMGT/DomainGapAlign results of the AZD8895 heavy and light chains with germ line V (IGHV1–58 and IGLV3–20), D (IGHD2–2, IGHD 2–8 or IGHD 2–15) or J (IGHJ3*02 and IGKJ1*01) gene segments and with representative variable gene sequences of mAbs in this public clonotype. Key interacting residues and their corresponding residues in germ line genes are highlighted in yellow and coloured in blue except for P99 in purple (heavy chain) or red (light chain). b, Binding curves of point mutants of AZD8895. Mutants of the D108 residue are in blue, GRev of inferred somatic mutations to germ line sequence are in green, P99 mutants are in orange and the C101A/C106A mutations removing the disulphide bond in HCDR3 are in purple. The data points show the mean ± s.d. for each tested antibody dilution. Experiments were performed in technical triplicate with data shown from a single experiment repeated twice. Data for the AZD8895 WT binding curve shown in both panels are from the same experiment.
