Extended Data Fig. 1: Receptor binding profile of avian and EA swine influenza A viruses.
An ELISA-based assay was employed to investigate receptor binding preference of avian and EA swine H1N1 influenza viruses. 3'SLN (shown in cyan circles) and 6'SLN (shown in pink squares) were coated in 96-well plates at concentrations of 5 to 0.1 µg/ml. Infectious viruses (diluted to 64 HAU/50µL) were added to each well and co-incubated with 3'SLN and 6'SLN. The plates were washed with PBS and the detection of bound virus was done using a rabbit polyclonal antibody against HA from A/Duck/NZL/160/1976(H1N3) (Sino Biological Inc., Beijing, China) followed by polyclonal Goat Anti-Rabbit Immunoglobulins/HRP (Dako, Denmark). The absorbance at 450 nm was plotted against glycan concentration. Results are shown with the mean and s.d. absorbance from 3 replicates in one of two independently performed experiment.
