Extended Data Fig. 2: EA swine virus sequence from archived pig-lung homogenates in 1979 shared high homology with the ancestral sequence of RG-EA2 that genetically resembles 1979–1983 era EA swine viruses. | Nature Microbiology

Extended Data Fig. 2: EA swine virus sequence from archived pig-lung homogenates in 1979 shared high homology with the ancestral sequence of RG-EA2 that genetically resembles 1979–1983 era EA swine viruses.

From: Ancestral sequence reconstruction pinpoints adaptations that enable avian influenza virus transmission in pigs

Extended Data Fig. 2

Full genome of A/swine/Belgium/2/1979 (Be02-lung) and a partial genome of A/swine/Belgium/1/1979 (Be01-lung) EA swine influenza viruses were sequenced from two archived pig-lung homogenates from 1979. Four recombinant viruses were resurrected using ancestral sequence reconstruction, with RG-EA1 representing the precursor virus of EA swine viruses, RG-EA2 representing the early evolutionary stage of EA swine viruses in 1979–1983, and RG-EA3 and RG-EA4 representing EA swine viruses in 1984–1987 and 1988–1992, respectively. Among the 33 amino acids that differentiate the non-transmissible RG-EA2 virus and transmissible RG-EA3 virus, the sequences of Be01 and Be02 showed high homology to RG-EA2. /, unsuccessfully determined.

Back to article page