Extended Data Fig. 3: The ecological effects of acrylate and DMSP on U. marinum.
From: Acrylate protects a marine bacterium from grazing by a ciliate predator
a–d, Cells stained with 5-(4,6-dichlorotriazinyl) aminofluorescein (DTAF). SM1211 (a), ΔdddL (b), ZH171 (c), and P. arctica (d). The images show that the cells are uniformly stained by DTAF and are similar in size. Scale bars = 5 μm. Each picture is a representative of at least three repeats. e, The chemotactic response of U. marinum to DMSP. The times of ciliates attracted into the microchannels were recorded for 10 min in the presence or absence of 500 μM DMSP (n = 1232 and 249, P value = 1.00E-06), and the ciliates detained in microchannels were counted after 10 min (n = 114 and 1, P value = 3.00E-04). The boxes in the plot are bound by the 25% to 75% quartile proportions with the thick blue line being the median value. Red dots indicate average values. A two-sided t-test was used to assess statistically significant differences between samples; ***, P < 0.001; n, sample size. f, The chemotactic response of U. marinum to DMS. The times of ciliates attracted into the microchannels were recorded for 10 min in the presence or absence of 100 μM DMS (n = 605 and 243, P value = 0.007), and the ciliates detained in microchannels were counted after 10 min (n = 57 and 12, P value = 0.005). A two-sided t-test was used to assess statistically significant differences between samples; **, P < 0.01; n, sample size. Data are presented as mean values +/- standard deviation. Error bars represent standard deviation of triplicate experiments. g, Growth curves of U. marinum preying on ΔdddL in the presence (grey) or absence (black) of 500 μM DMS. h, i, Growth curves of U. marinum preying on strain SM1211 (h) and its mutant ΔdddL (i) in the presence (grey) or absence (black) of 70 μM acrylate. The dotted red lines indicate the concentration of acrylate in the system with DMSP. No acrylate was detected in the system without DMSP. The acrylate concentration on the y-axis is shown in red. All experiments were carried out in technical triplicates. j, Effect of the addition of 0 (red), 70 μM (blue), 500 μM (orange) or 1 mM (grey) acrylate on the growth of U. marinum. Inactivated P. arctica was used as prey for U. marinum. No acrylate was detected in the system without acrylate. All experiments were carried out in technical triplicates.
