Fig. 3: Male cifB expression is sufficient to cause CI, while male cifA attenuates it.
From: Wolbachia cifB induces cytoplasmic incompatibility in the malaria mosquito vector
a, zpg-cifB males caused infertility in WT females, while zpg-cifA males did not (Dunn’s multiple comparisons test (two-sided), P ≤ 0.0001 for differences between all statistical groups). Median and interquartile ranges are shown. For each group (top to bottom), the n is as follows: 55, 55, 44, 39. Kruskal–Wallis test: H = 133.8, P < 0.0001, d.f. = 3. b, The expression of vasa-cifA in females rescued infertility caused by (v)zpg-cifB expression in males, which induced CI to the same extent as (z)zpg-cifB males (Dunn’s multiple comparisons test (two-sided), P ≤ 0.0001 for differences between all statistical groups). Median and interquartile ranges are shown. For each group (top to bottom), the n is as follows: 36, 39, 24, 32. Kruskal–Wallis test: H = 95.08, P < 0.0001, d.f. = 3. c, Expression of vasa-cifA;zpg-cifB in males caused only partial induction of CI (Mann–Whitney test (two-tailed), P < 0.0001). Median and interquartile ranges are shown. For each group (top to bottom), the n is as follows: 18, 34. d, Expression of cifA in the male germline was higher in vasa-cifA than in zpg-cifA (unpaired t-test (two-tailed), *P = 0.0135, mean and s.d. are shown), while the expression of cifB was similar (unpaired t-test (two-tailed), P = 0.4882, mean and s.d. are shown). For each group (left to right), the total n is as follows: 32, 48, 32, 48.
