Fig. 4: cifB expression in females causes severely impaired follicle development in the absence of cifA.
From: Wolbachia cifB induces cytoplasmic incompatibility in the malaria mosquito vector
a, Crosses were set up to isolate zpg-cifB females, F1 progeny derived from either mothers that also expressed vasa-cifA ((vmat)zpg-cifB) or zpg-cifA ((zmat)zpg-cifB), or fathers also expressing vasa-cifA ((vpat)zpg-cifB). b, Egg development was nearly abolished in (zmat)zpg-cifB-expressing females, while nearly all (vmat)zpg-cifB females showed egg development, although with decreased numbers of eggs compared with WT females (Dunn’s multiple comparisons test (two-sided), P < 0.0001 for differences between all statistical groups). Medians and interquartile ranges are shown. For each group (top to bottom), the n is as follows: 68, 66, 68. Kruskal–Wallis test: H = 153.9, P < 0.0001, d.f. = 2. c, (zmat)zpg-cifB and (vmat)zpg-cifB females showed impaired fertility compared with WT females (Dunn’s multiple comparisons test (two-sided), P < 0.0257 between groups b and c, P < 0.0001 for other comparisons). Median and interquartile ranges are shown. For each group (top to bottom), the n is as follows: 60, 54, 17. Kruskal–Wallis test: H = 64,64, P < 0.0001, d.f. = 2. d,e, Ovaries from cifB females showed severely impaired follicle development unless derived from a vasa-cifA-expressing mother, when imaged at either 0 h or 24 h post blood feeding (p.b.f.) before fixing under brightfield microscopy (d) (scale bar, 800 μm) or after fixing using differential interference contrast microscopy (e) (scale bar, 100 μm).
