Extended Data Fig. 4: F14 does not inhibit the nuclear translocation of NF-κB subunit p65. | Nature Microbiology

Extended Data Fig. 4: F14 does not inhibit the nuclear translocation of NF-κB subunit p65.

From: Molecular mimicry of NF-κB by vaccinia virus protein enables selective inhibition of antiviral responses

Extended Data Fig. 4

a, T-REx-293 cells inducibly expressing the empty vector (EV) or VACV proteins B14, C6, or F14, were induced with doxycycline and stimulated with TNF-α. Fixed and permeabilised cells were stained with anti-p65 antibody and DAPI, and analysed by confocal microscopy. Scale bars (50 μm) are shown in the bottom right of each micrograph. Representative micrographs of quantitative analysis shown in Fig. 1l. b, Flow cytometry analysis of T-REx-293-F14 induced with doxycycline in the absence and in the presence of the proteasome inhibitor MG132. F14 presence was detected by staining with an anti-FLAG antibody.

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