Extended Data Fig. 5: F14 inhibits NF-κB at or downstream of p65.

NF-κB activity in HEK 293 T cells transfected with vectors expressing p65, VACV proteins B14 or F14, or empty vector (EV). Top panel: Means + s.d. (n = 4 per condition) are shown. Statistical significance was determined by unpaired two-tailed Student’s t-test. Bottom panel: Immunoblotting. Protein molecular mass markers in kDa are shown on the left of the blots. Immunoblots of tagged proteins are labelled with the protein name followed by the epitope tag antibody in parentheses. When multiple tagged proteins are shown in the same immunoblot, each protein is indicated by a red arrowhead.