Extended Data Fig. 9: Target site locus and strain comparisons for selective enrichment from the infant gut community.
From: Species- and site-specific genome editing in complex bacterial communities
a, Clinically relevant gene clusters targeted by VcDART for selective enrichment included a locus associated with fimbriae biosynthesis (top) and a propanediol utilization gene cluster (bottom). Insets show mapped reads to these loci in E. coli subsp. 2 and subsp. 3, which were assembled from enrichment culture shotgun sequencing data. The right end of the VcDART transposon cargo was assembled (green), is bridged to the genome, and is supported by paired end read mapping. VcDART target sites (protospacer) are indicated in dark red. b, Dendrogram displaying average nucleotide identity differences between all E. coli genomes analyzed as part of the infant gut community. Strains in black were genomes originally recovered from metagenomic assembly in Lou, et al. 2021. Strains in red were assembled out of enrichment cultures in this study.
