Extended Data Fig. 10: Location of VcDART transposon insertions in isolated E. coli mutant colonies following infant gut community editing.
From: Species- and site-specific genome editing in complex bacterial communities
a, Insertion orientations and locations relative to target site were determined by locus-specific PCR and Sanger sequencing on colonies picked from selective solid medium after editing the infant gut community with VcDART guided by the fimbriae associated locus-targeting guide RNA and b, the propanediol metabolism locus-targeting guide RNA (n = 3 biological replicates).
