Fig. 5: SDSI + AmpSeq is used to identify sample swaps and contamination. | Nature Microbiology

Fig. 5: SDSI + AmpSeq is used to identify sample swaps and contamination.

From: Synthetic DNA spike-ins (SDSIs) enable sample tracking and detection of inter-sample contamination in SARS-CoV-2 sequencing workflows

Fig. 5

a, Intentional SDSI contamination experiment (run in duplicate) assessing whether different ratios of contamination between SDSI 87 and SDSI 94 (SDSI 87:SDSI 94) were detectable with the SDSI + AmpSeq method. b, Examples of experimental errors that were caught using the SDSI + AmpSeq method. c, Top: distance matrix showing pairwise differences between the 17 complete genomes assembled from this sample set. Putative cluster samples are in bold. Bottom: spike-in counts for each of the 24 samples and water controls in this sequencing batch.

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