Extended Data Fig. 4: ARTIC SARS-CoV-2 amplicon sequencing with and without SDSI and normalization. | Nature Microbiology

Extended Data Fig. 4: ARTIC SARS-CoV-2 amplicon sequencing with and without SDSI and normalization.

From: Synthetic DNA spike-ins (SDSIs) enable sample tracking and detection of inter-sample contamination in SARS-CoV-2 sequencing workflows

Extended Data Fig. 4

a, In three different CT bins, we show coverage plots with 95% confidence intervals for multiple samples sequenced with and without SDSIs (CT < 27, n = 4; CT 27-29, n = 6; CT > 30, n = 4). The solid blue line represents SDSI + AmpSeq and the solid black line is ARTIC only with no SDSI. Blue and black shading around the solid lines represents the 95% confidence interval. There were no significant differences (p-value > 0.05) between the with and without SDSI group for the mean coverage at any of the amplicons (two-tailed Mann Whitney t-test and multiple comparison two-stage step-up Benjamini, Krieger, and Yekutieli test with FDR set to 5%). b, The percentage of SDSI reads for 4 different SDSIs was assessed within 4 clinical samples that were run with and without CT normalization of the cDNA prior to the ARTIC PCR.

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