Extended Data Fig. 1: The predicted DNA-binding protein HDP1 is expressed in gametocytes. | Nature Microbiology

Extended Data Fig. 1: The predicted DNA-binding protein HDP1 is expressed in gametocytes.

From: The transcriptional regulator HDP1 controls expansion of the inner membrane complex during early sexual differentiation of malaria parasites

Extended Data Fig. 1

(a) The single exon locus Pf3D7_1466200 encodes a large 3078aa protein with a predicted C-terminal Helix-Turn-Helix DNA-binding domain (DBD) and two nuclear localization signals (NLS). Multiple AP2-G binding sites are found in its 2 kb promoter region. Black bracket indicates the antigen used for generating antisera used in29. (b) Alignment of the helix-turn-helix domain for homologs from other apicomplexan parasites. (c) Quantitative RT-PCR of hdp1 transcripts found minimal expression in asexual blood stages with upregulation during gametocyte development (data are presented as mean values of n = 2 biologically independent samples). Transcript levels were normalized to Seryl-tRNA synthetase.

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