Extended Data Fig. 2: Validation of engineered parasite lines.
(a) Generation of halo-hdp1 parasites by CAS9 genome editing. Insertion of the N-terminal HALO tag at the 5’ end of hdp1 coding sequence was confirmed by PCR and checked for mutations by Sanger sequencing of the 3.8 kb PCR product (not shown). (b) Generation of hdp1-gfp parasites by CAS9 genome editing. Insertion of the C-terminal GFP tag at the 3’ end the hdp1 coding sequence was confirmed by PCR and checked for mutations by Sanger sequencing of the 1.9 kb PCR product (not shown). (c) Generation of hdp1-Ty1 parasites by CAS9 genome editing. Insertion of the C-terminal triple Ty1 epitope tag at the 3’ end the hdp1 coding sequence was confirmed by PCR and checked for mutations by Sanger sequencing of the 1.1 kb PCR product (not shown). (d) Generation of ∆hdp1 parasites by CAS9 genome editing. Replacement of 1.4 kb flanking the hdp1 start codon by a hDHFR selectable marker cassette was confirmed by PCR. (e) Generation of hdp1-glmS parasites by CAS9 genome editing. Insertion of the C-terminal triple Ty1 epitope tag and the glmS ribozyme at the 3’ end the hdp1 coding sequence was confirmed by PCR and checked for mutations by Sanger sequencing of the 1.4 kb PCR product (not shown).
