Extended Data Fig. 8: Determining the impact of Bacteroides species on TEER using co-culture, supernatants and protease inhibitors.
a, Bacteroides vulgatus and Bacteroides dorei, but not other Bacteroides species disrupt Caco-2 epithelial barriers. Barplots are showing the mean and standard deviation of the change in TEER at different time points. Data was derived from n = 3 independent cultures collected over n = 2 independent experiments. b, Growth curves of Bacteroides vulgatus with protease inhibitors under different growth conditions. OD600 was measured at indicated time points and a non-linear fit is shown. Data was derived from n = 3 independent cultures collected over n = 1 independent experiments. c, Supernatants from Bacteroides in mid-log phase growth do not significantly impact TEER. B. vulgatus and B. theta were grown to mid-log phase, and their supernatants were concentrated and added to Caco-2 monolayers. TEER was measured at the initial time-point and compared to TEER measured after 1, 4, and 8 h of incubation. Plotted are the mean and SEM from n = 3 independent experiments each representing the mean of n = 3 independent wells/experiment (n = 4 wells/experiment for B. vulgatus group). No significant differences were found at any timepoint.
