Extended Data Fig. 6: FGT Lactobacillus genomes lack predicted alternate Cys and GSH transporters and L. iners is selectively inhibited by cystine uptake inhibitors.
a, Predicted presence of the branched-chain amino acid transport locus livFGHKM (which has low-affinity Cys transport activity in E. coli) and the glutathione transport locus gsiABCD in isolate genomes and MAGs of common FGT Lactobacillus species (n = number of genomes. Detailed statistics are in Supplementary Table 12). b, Selective growth inhibition of L. iners in MRSQ broth with or without L-Cys (4 mM) and varying concentrations of the cystine uptake inhibitor seleno-DL-cystine (SDLC). c, Growth of L. crispatus and L. iners in HMRSQ broth with or without L-Cys (4 mM) ± SMC. d, Growth inhibition of L. crispatus and L. iners by SMC (128 mM) or SDLC (2 mM) in MRSQ supplemented with L-cystine (2 mM) or D-Cys, TCEP, or GSH (4 mM each). For each growth additive, percentage growth in in presence of inhibitor was calculated relative to median growth in broth containing that additive without inhibitor. Plots in b-d depict median ± range for 3 replicates per condition and each plot is representative 1 of ≥2 independent experiments per strain and media condition.
