Extended Data Fig. 7: Sample preparation and controls for L. iners / L. crispatus growth competition assays.
a, Mono-culture growth of L. crispatus (strain FRESH1) and 3 representative L. iners strains at 28 hrs incubation in MRSQ broth with or without L-Cys (4 mM) and varying concentrations of the cystine uptake inhibitor S-methyl-L-cysteine (SMC), exhibiting expected growth patterns for the respective species. Plots depict median ± range for 3 replicates per condition and each plot is representative 1 of ≥2 independent experiments per strain and media condition. The competition assays between L. iners and L. crispatus depicted in Fig. 5a were prepared by mixing the input inocula from each of these L. iners monocultures pairwise with the input inoculum for the L. crispatus monoculture at the colony-forming unit (c.f.u.) ratios listed in the legend of Fig. 5a. b, Bacterial 16 S rRNA gene read counts in cultured samples (n = 105 individual cultures), with negligible background read counts in blank growth media controls (n = 7 controls, one each per media type) and extraction controls (n = 7 controls) associated with the competition experiments in main Fig. 5a. (Control sample reactions were included in the sequencing library despite absence of visible PCR bands on agarose gel electrophoresis.) Box center lines, edges, and whiskers signify the median, IQR, minima and maxima respectively.
