Extended Data Fig. 8: Development of “S-broth” and controls for mock BV-like community growth experiments.
a, Monoculture growth at 48 hours of experimental US (strain “233”) and South African (strains FRESH1 and FRESH2) L. crispatus strains, as well as L. iners, G. vaginalis, Prevotella bivia, Prevotella disiens, and Sneathia sanguinegens strains in various broth media including MRSQ broth + L-Cys (4 mM), NYCIII broth with or without 2% IsoVitaleX plus 5% Vitamin K1-Hemin solution (“IHK”) and/or Tween-80 (1 g/L), and in “S-broth” (see Methods) with or without Tween-80 (1 g/L). S-broth + Tween was used in subsequent experiments. (Detailed strain information is in Supplementary Table 3). Plots depict median ± range for 3 replicates per condition and each plot is representative 1 of ≥2 independent experiments per strain and media condition. b, Bacterial 16 S rRNA gene read counts in mock mixed communities (n = 72 individual cultures) and pure strain monocultures (n = 7), with negligible background read counts in blank growth media controls (n = 4 controls, one each per media type) and extraction controls (n = 2 controls) associated with the experiments in Fig. 5c,d. (Control sample reactions were included in the sequencing library despite absence of visible PCR bands on agarose gel electrophoresis.) Box center lines, edges, and whiskers signify the median, IQR, minima and maxima respectively. c, Confirmation of identity of input strains in mock BV-like communities by 16 S rRNA gene sequencing of bacterial monoculture controls, prepared from the same input inocula used for the bacterial mock communities shown in Fig. 5c,d.
