Extended Data Fig. 1: L-cysteine supplementation supports L. iners growth in Lactobacillus MRS broth, augmented by L-glutamine.
a,b Growth of L. crispatus, L. iners, and G. vaginalis at 24 hours incubation in MRS broth (BD DIFCO) ± supplementation with 2% IsoVitaleX (“Iso”) or with the indicated sub-pools of IsoVitaleX components. c, Growth in MRS broth supplemented with 2% IsoVitaleX or various combinations of the nutrients in “Pool 2”. d, Growth of L. iners at 24 hours in MRS broth + L-Gln (1.1 mM) supplemented with varying concentrations of L-Cys or e, in MRS broth + L-Cys (4 mM) supplemented with varying concentrations of L-Gln. Experiments in (a-e) all used BD DIFCO-formulated MRS broth base. (f) Growth of L. iners in Hardy Criterion-formulated MRS (“HMRS”) broth supplemented with IsoVitaleX 2% v/v, L-Cys (4 mM), and/or L-Gln (1.1 mM) produced similar results, although with a substantially longer lag phase. Each plot depicts median (± range) for 3 replicates per condition and each plot is representative 1 of ≥2 independent experiments per strain and media condition except (a), which was un-replicated.
